Plasmid_Backbone
Part:BBa_K4161302:Design
Designed by: Qizhou Jiang Group: iGEM22_DKU (2022-07-25)
pyD1 plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal XbaI site found at 975
Illegal XbaI site found at 2019
Illegal PstI site found at 801
Illegal PstI site found at 941 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal NheI site found at 858
Illegal PstI site found at 801
Illegal PstI site found at 941
Illegal NotI site found at 962 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal BamHI site found at 921
Illegal XhoI site found at 969 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal XbaI site found at 975
Illegal XbaI site found at 2019
Illegal PstI site found at 801
Illegal PstI site found at 941 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal XbaI site found at 975
Illegal XbaI site found at 2019
Illegal PstI site found at 801
Illegal PstI site found at 941
Illegal AgeI site found at 71
Illegal AgeI site found at 1038 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1020
Illegal BsaI site found at 3646
Illegal SapI.rc site found at 2624
Illegal SapI.rc site found at 4728
Design Notes
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Source
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